Innovative design and comprehensive characterization of a valine-enhanced lipid nanoparticle-coated mRNA vaccine targeting the SARS-CoV-2 S-RBD antigen.

ABSTRACT: The SARS-CoV-2 pandemic has led to major advances in mRNA vaccine development. This study aimed to develop a cationic lipid nanoparticle (LNP) coated mRNA vaccine targeting the receptor binding domain (RBD) against the SARS-CoV-2 virus and adding valine to the N-terminal end to increase its stability, and to comprehensively characterize the physical, thermal, microscopic and biological properties of vaccine. The vaccine was designed in silico and valine-added S-RBD molecule was generated by IVT. The LNP coated mRNA vaccine was evaluated by spectroscopic (UV-vis, FT-IR, Raman), thermodynamic (TGA/DSC), microscopic (STEM, AFM), size/potential (DLS) and surface tension analysis methods. The developed vaccine was analyzed for cytotoxicity (MTT) and pro/anti-inflammatory gene expression (qPCR).

SUBMITTER: Duran T 

PROVIDER: S-EPMC12484943 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature