Cardiotrophin-like cytokine factor 1 forms a complex with IL12/IL23p40.

ABSTRACT: Cardiotrophin-like cytokine factor 1 (CLCF1) is a cytokine of the IL6/IL12 superfamily with pro-neurotrophic and immune-modulating functions. Although the pro-neurotrophic activities of CLCF1 are mediated through the ciliary neurotrophic factor receptor (CNTFR), the α receptor chain of the CNTFR, CNTFRα, is not expressed by immune cells. This suggests the presence of an alternative receptor or protein complex that mediates the immune activities of CLCF1. Using the BioID2 proximity-dependent biotinylation assay, we identified p40, the β subunit of IL12/IL23, as a potential interaction partner for CLCF1. We confirmed the protein-protein interaction between CLCF1 and p40 using co-immunoprecipitation and a proximity ligation assay. We also observed that the CLCF1-p40 complex forms both intrace

SUBMITTER: Laplante V 

PROVIDER: S-EPMC12485051 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature