Single molecule spectrum dynamics imaging with 3D target-locking tracking.

ABSTRACT: Fluorescence spectra offer rich physicochemical insights into molecular environments and interactions. However, imaging the dynamic fluorescence spectrum of rapidly moving biomolecules, along with their positional dynamics, remains a significant challenge. Here, we report a three-dimensional target-locking-based single-molecule fluorescence Spectrum Dynamics Imaging Microscopy (3D-SpecDIM), a method capable of simultaneously capturing both rapid 3D positional dynamics and physicochemical parameter changing dynamics of the biomolecules with enhanced spectral accuracy, high spectral acquisition speed, single-molecule sensitivity, and high 3D spatiotemporal localization precision. As a demonstration, 3D-SpecDIM is applied to real-time spectral imaging of the mitophagy process, highlighting it

SUBMITTER: Sha H 

PROVIDER: S-EPMC12485062 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature