M464 inhibits activation of NLRP3 inflammasome and inflammatory response in mice.

ABSTRACT: Non-steroidal anti-inflammatory drugs (NSAIDs) function as antipyretic, analgesic and anti-inflammatory agents. However, its long application might result in gastrointestinal side effects. Therefore, it is imperative to hunt for novel and safer NSAIDs. Here, we report a new class of NSAIDs, M464, which was equipped with a hydrogen sulfide-releasing radical. We delved into its anti-inflammatory properties and underlying molecular mechanisms. In vitro, the relevance of the anti-inflammatory effects of M464 through the NLRP3 signaling pathway was explored by lactate dehydrogenase (LDH) release, WB (Western Blotting), ELISA assays and immunofluorescence experiments. In vivo, the influence and correlation of M464 on liver and lung tissue injury were investigated by means of histological evaluat

SUBMITTER: Wang W 

PROVIDER: S-EPMC12485066 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature