Prominent involvement of acetylcholine dynamics in stable olfactory representation across the Drosophila brain.

ABSTRACT: Despite the vital role of neuromodulators and neurotransmitters in the neural system, their spatiotemporal correlation with neuronal activities across multiple brain regions remain unclear. Here, we employed two-photon synthetic aperture microscopy (2pSAM) and neurochemical indicators to simultaneously record calcium and acetylcholine (ACh)/5-HT dynamics across multiple regions of the Drosophila brain over 2 h. Presenting 3 different odors across multiple trials, our analyses revealed signal-specific differences in responsiveness, functional connectivity, and odor classification accuracy across the brain. We further constructed low-dimensional manifolds to characterize the global odor-related dynamics. Incorporating both calcium and ACh signals enhanced odor classification accuracy in the

SUBMITTER: Fan J 

PROVIDER: S-EPMC12485117 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature