Design and evolution of artificial enzyme with in-situ biosynthesized non-canonical amino acid.

ABSTRACT: The creation of designer enzymes by incorporating genetically encoded non-canonical amino acids (ncAAs) could significantly expand the catalytic repertoire of the enzyme universe for abiological transformations. However, due to the limited availability of ncAAs with potential catalytic functional groups, progress in this field remains relatively slow. Herein, we present an efficient approach for enzyme design with organocatalytic ncAAs by integrating the biosynthesis and genetic incorporation of ncAAs harboring abiological catalytic residues into the protein scaffold. Based on this ncAAs in situ biosynthesis and incorporation system, our designer enzyme with an unnatural mercapto-aniline residue is created efficiently by feeding thiols to E. coli and shows significant catalytic activity fo

SUBMITTER: Huang W 

PROVIDER: S-EPMC12485135 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature