Induction of experimental cell division to generate cells with reduced chromosome ploidy.

ABSTRACT: Somatic cell nuclear transfer (SCNT) enables the direct reprogramming of somatic cells into functional oocytes, albeit with a diploid genome. To address ploidy reduction, we investigated an experimental reductive cell division process, termed mitomeiosis, wherein non-replicated (2n2c) somatic genomes are prematurely forced to divide following transplantation into the metaphase cytoplasm of enucleated human oocytes. However, despite fertilization with sperm, SCNT oocytes remained arrested at the metaphase stage, indicating activation failure. Artificial activation using a selective cyclin-dependent kinase inhibitor successfully bypassed this arrest, inducing the segregation of somatic chromosomes into a zygotic pronucleus and a polar body. Comprehensive chromosome tracing via sequencing rev

SUBMITTER: Marti Gutierrez N 

PROVIDER: S-EPMC12485136 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature