Androgen Receptor-Controlled microRNA-124-3p.2 Suppresses Prostate Cancer Progression via CCL2 Inhibition.

ABSTRACT: We previously reported that androgen receptor (AR) signaling blockade induces chemotactic-C-C-motif-chemokine-ligand-2 (CCL2) secretion from prostate cancer cells and activates cancer cells through an autocrine manner. However, the mechanism of how AR negatively regulates CCL2 expression is still unclear. As various microRNAs participate in prostate cancer development, we hypothesized that there are AR-controlled miRs that regulate CCL2 production. Using LNCaP and C4-2B cells, miR-124-3p.2 was found as a potential miR from the miRNA PCR array and public database. The expression of miR-124-3p.2 was inhibited by knockdown AR, and the introduction of miR-124-3p.2 mimic decreased CCL2 expression and suppressed cell migration. Dihydrotestosterone led to miR-124-3p.2 upregulation and CCL2 downre

SUBMITTER: Aoyama S 

PROVIDER: S-EPMC12485657 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature