Interaction of class III cellobiose dehydrogenase with lytic polysaccharide monooxygenase.

ABSTRACT: The genome of Fusarium solani, a well-known plant pathogen, encodes various lytic polysaccharide monooxygenases (LPMOs) involved in plant biomass degradation in combination with cellobiose dehydrogenase (CDH). To investigate the auxiliary role of the recently expressed and characterized class III CDH from F. solani (FsCDH), this enzyme was tested in combination with the well-characterized AA9C from Neurospora crassa (NcAA9C). Steady-state and stopped-flow methods as well as electrochemical measurements demonstrate how FsCDH efficiently transfers electrons to NcAA9C, with a rapid, observed heme reoxidation rate constant of 129 s^-1. In comparison to ascomycete class II CDHs, the H₂O₂ production by FsCDH is insufficient to promote LPMO activity. However, a cyc

SUBMITTER: Giorgianni A 

PROVIDER: S-EPMC12485664 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature