Differentiation stage-specific use of cap-independent and cap-dependent translation initiation in hematopoiesis.

ABSTRACT: Cell stress can increase the use of m⁷G-cap-independent, IRES-mediated translation initiation relative to cap-dependent translation (IRES/Cap). Reporters that quantify IRES/Cap have demonstrated differential activity across cultured cell types and stress conditions. By generating an IRES/Cap reporter mouse, we were able to systematically evaluate IRES/Cap across distinct tissues and cell types during physiological stresses and lineage commitment. Caloric stress invoked the expected boost in IRES/Cap translation regardless of differentiation state, but unexpectedly IRES/Cap progressively increased during hematopoietic and epithelial (hair follicle) differentiation under normal, homeostatic conditions. This was independent of total protein output or cell cycle. Even within cells o

SUBMITTER: Mazzola MC 

PROVIDER: S-EPMC12485689 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature