Microbial lipid shifts in a multi-stage simulated gut.

ABSTRACT: Food residues that bypass human digestion are further digested by gut microbes, leading to the production of diverse metabolites, including lipids. To investigate how lipids are affected during this transition, we used a colon simulator with four distinct vessels that mimics the proximal to distal part of the human colon. We observed dynamic shifts in a diverse array of microbially derived lipid molecules in the simulated intestinal chyme, including bile acids and N-acyl amides with short and odd-chain lipids. Histamine-linked N-acyl lipids increased from the proximal to the distal colon vessels (pH 5.5 - 7.0), whereas putrescine-linked, initially abundant in the media, decreased across the colon vessels. We uncovered dynamic associations between in vitro-derived short-chain

SUBMITTER: Tamanna I 

PROVIDER: S-EPMC12485695 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature