Pseudocapillaria tomentosa Infections in Laboratory Larval and Adult Zebrafish (Danio rerio): Development and Advances in an In Vivo Anthelmintic Drug Discovery Model.

ABSTRACT: Parasite resistance is an increasing problem in livestock and companion animals. Developing new drug discovery models may improve the identification of novel anthelmintic drugs which will reduce parasite infections. Adult zebrafish (Danio rerio) have been previously used as a model for anthelmintic drug discovery by infecting them with the gastrointestinal nematode Pseudocapillaria tomentosa. Using larval zebrafish will increase assay sensitivity and throughput because this in vivo platform evaluates host parasite interactions and is conducted in multi-well plates. To develop this assay, this study focuses on 1) evaluating infections in 5-30 days post fertilization (dpf) fish, 2) validating the assay with a known anthelmintic used in domestic animals, and 3) documentin

SUBMITTER: Leong C 

PROVIDER: S-EPMC12485721 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature