Computational method for mapping mass signatures along developmental gradients reveals a novel role for a monosaccharide tetrose in maize salt-stress response.

ABSTRACT: Metabolic processes are essential for regulating and maintaining developmental transitions, from stem cell quiescence through differentiation. However, the distinct metabolite-driven mechanisms that are critical for development remain poorly characterized due to inherent challenges in measuring their production, localization, and function in situ. We employed desorption electrospray ionization mass spectrometry imaging (DESI-MSI) to map metabolites in the developing maize root, which has a well-characterized longitudinal gradient that encompasses developmental transitions from quiescence through proliferation and maturation. DESI-MSI enables in situ analysis of the chemical composition of tissue sections with high spatial resolution (~50-100 μm). To identify metabolites with

SUBMITTER: Sama AM 

PROVIDER: S-EPMC12485800 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature