Mining thermophile photosynthesis genes: a synthetic operon expressing <i>Chloroflexota</i> species reaction center genes in <i>Rhodobacter sphaeroides</i>.

ABSTRACT: Photosynthesis is the foundation of the vast majority of life systems, and therefore the most important bioenergetic process on earth, and the greatest diversity in photosynthetic systems are found in microorganisms. However, understanding of the biophysical and biochemical processes that transduce light to chemical energy has derived from the relatively small subset of proteins from microbes that are amenable to cultivation, in contrast to the huge number of microbial DNA sequences encoding proteins that catalyze the initial photochemical reactions that has been deposited in databases, such as from metagenomics. We describe the use of a Rhodobacter sphaeroides laboratory strain for expression of heterologous photosynthesis genes to demonstrate the feasibility of mining this resourc

SUBMITTER: Rehman Y 

PROVIDER: S-EPMC12485845 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature