Aurora-A Promotes Cell-Cycle Progression From Quiescence Through Primary Cilia Disassembly.

ABSTRACT: Aurora-A (AurA) is a member of the mitotic kinase family and is highly expressed in various tumors. Inhibition of AurA generally leads to fetal mitotic errors. We previously reported that AurA inhibition induces G₀/G₁ cell cycle arrest in noncancerous cells by promoting the reassembly of primary cilia. However, the mechanisms by which AurA regulates cell cycle progression beyond mitosis remain largely unknown. In this study, we generated noncancerous RPE1 and cancerous HCT116 cell lines expressing endogenous AurA tagged with a minimal auxin-inducible degron (mAID) using CRISPR/Cas9-based gene editing. This system enabled specific and rapid depletion of endogenous AurA protein. By combining this approach with cell synchronization in RPE1 cells, we investigated AurA fun

SUBMITTER: Kohso A 

PROVIDER: S-EPMC12485896 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature