Self-organization of sinusoidal vessels in pluripotent stem cell-derived human liver bud organoids.

ABSTRACT: The induction of tissue-specific vessels in in vitro living tissue systems remains challenging. Here, we directly differentiated human pluripotent stem cells into CD32b⁺ putative liver sinusoidal progenitors by dictating developmental pathways. By devising an inverted multilayered air-liquid interface culture, hepatic endoderm, septum mesenchyme, arterial and sinusoidal quadruple progenitors self-organize to generate and sustain hepatocyte-like cells neighboured by divergent endothelial subsets composed of CD32b^lowCD31^high, LYVE1⁺STAB1⁺CD32b^highCD31^lowTHBD^-vWF^- and LYVE1^-THBD⁺vWF⁺ cells. WNT2 mediates sinusoidal-to-hepatic intercellular crosstalk potentiating

SUBMITTER: Saiki N 

PROVIDER: S-EPMC12486625 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature