Streamlined purification of ADDomer nanoparticles for scalable biomanufacturing.

ABSTRACT: Current lab-scale purification methods for ADDomer are labour-intensive, time-consuming and poorly scalable. The intracellular nature of ADDomer production further complicates downstream processing, requiring robust, scalable solutions for cell lysis and clarification. In the present work we focus on developing a scalable, GMP-compliant process for ADDomer purification. The workflow combines tangential microfiltration (TMF) using a 0.4 μm hollow fiber for cell retention, chemical lysis with 0.06 % Deviron^® C16, and in-line Benzonase^® treatment for DNA removal. Several capture approaches were explored, including anion and cation exchange, hydrophobic interaction and multimodal chromatography. Among these, the Sartobind^® Q anion exchange membrane demonstrated

SUBMITTER: Arinto-Garcia R 

PROVIDER: S-EPMC12487075 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature