Changji'an formula alleviates visceral hypersensitivity of a post-inflammatory IBS-D mouse model via NGF/TrkA signaling pathway.

ABSTRACT:

Background

Changji'an Formula (CJAF) is an effective Chinese herbal prescription to treat irritable bowel syndrome with predominant diarrhea (IBS-D), which is derived from two famous classical prescription: Sijunzi decoction and Tong-Xie-Yao-Fang. However, the molecular mechanism has not been well defined. This study aims to illustrate the molecular mechanism of CJAF in the treatment of IBS-D.

Methods

Chemical components of CJAF were determined by ultrahigh performance liquid chromatography-quadrupole/orbitrap high resolution mass spectrometry (UPLC-Q-Orbitrap HRMS) and further verified by reference standards. IBS-D model was induced in C57BL/6J mice by a single edema with colonic infusion of 0.1 mL trinitrobenzene sulfonic acid (TNBS, 50 mg/mL) combined with 7 days of restr

SUBMITTER: Ke W 

PROVIDER: S-EPMC12487093 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature