Dielectrophoresis-assisted microfluidic device for high-precision and periodic single-cell capture and release.

ABSTRACT: Single-cell analysis is crucial for understanding the specificity of individual cells, yet its advancement is limited by the technical challenges of precise single-cell manipulation. Microfluidics has made significant advancements in single-cell manipulation, making it a powerful tool for analysis. This study presents a dielectrophoresis-assisted microfluidic device for single-cell manipulation. By coupling flow and electric fields, this device enables single-cell focusing, along with fixed-frequency capture and release on a microfluidic chip. This study employs theoretical and finite element method analysis to determine the cell dielectric parameters (K562), medium dielectric parameters (σ_m = 55 mS/m, ε_m = 7.08 × 10^-10), flow field parameters, and electric

SUBMITTER: Yu Z 

PROVIDER: S-EPMC12487163 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature