A new in vitro model of the adult pig colon microbiome: application to the study of feed ingredients.

ABSTRACT:

Background

There is an intricate relationship between pig nutrition and gut microbiome. For technical, cost and societal reasons, in vitro gut models can be advantageously used as an alternative to in vivo experiments to perform mechanistic studies on the interactions between gut microbes and nutrients. In this context, the aim of the present study was to develop a new in vitro model of the healthy adult pig colon integrating both luminal and mucosal microenvironments. The model was further used to evaluate feed ingredients (lactose and lactose + sucrose). Gut Microbiota composition and metabolic activities were followed by 16S Metabarcoding and short chain fatty acid (SCFA) / gas measurement, respectively. Then, the effect of the both feedstuffs on skatole production, responsible

SUBMITTER: Durif C 

PROVIDER: S-EPMC12487194 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature