NRF2 promotes the migration of ovarian cancer cell lines by targeting TAGLN mediated epithelial-mesenchymal transition.

ABSTRACT:

Objective

Migration is one of the essential steps of cancer cell metastasis. Here we try to demonstrate the molecular crosstalk between NRF2-TAGLN pathway and ovarian cancer migration.

Methods

Western blot and Real-time PCR were used to determine the expression of NRF2 and TAGLN. Pharmacological treatment and gene intervention were employed to modulate gene expression. Wound-healing and Transwell assay were used to examine the migration ability of ovarian cancer cell lines. Dual luciferase activity and chromatin immunoprecipitation assay (CHIP) were applied to verify TAGLN as a NRF2 target gene.

Results

NRF2 overexpression promoted the migration of ovarian cancer cells. Our previous microarray data indicated 18 putative NRF2 target genes. Among these genes, TAGLN, bin

SUBMITTER: Wang H 

PROVIDER: S-EPMC12487345 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature