Chloroplast genome, an effective strategy for identifying hybrid species, using Dendrobium 'Black Gold' as an example.

ABSTRACT:

Background

Misusing Dendrobium hybrid varieties in herbal products poses a substantial challenge to authenticity and safety concerns. D. 'Black Gold', a hybrid of D. nobile and D. officinale, closely mimics D. officinale in appearance. However, the accurate identification of genetic traits has been impeded by the scarcity of genetic data. Molecular markers derived from chloroplast genomes (cp genomes) have emerged as practical tools for authenticating cultivars.

Results

In this study, three Dendrobium species (D. 'Black Gold', D. nobile, and D. officinale) cp genomes were de novo sequenced, assembled, And characterized. The Analysis revealed high conservation in gene organization and structure across the three genomes. The genome sizes ranged from 150,114 bp to 150,602 bp, w

SUBMITTER: Li C 

PROVIDER: S-EPMC12487424 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature