Hypoxia-inducible factor-2 stabilization is not sufficient to induce erythropoietin production in deeper medullary fibroblasts.

ABSTRACT: Under hypoxaemic conditions, cortical fibroblasts primarily produce erythropoietin (EPO). However, we have previously shown that most interstitial fibroblasts positive for platelet-derived growth factor receptor β (PDGFR-β) in all kidney zones are also able to produce EPO. Therefore, we wondered if either the physiological stimuli might not be sufficient to stabilize the hypoxia-inducible factor (HIF)-2 in medullary fibroblasts or if different expression patterns or functions of the HIF-regulating prolyl-4-hydroxylases (PHD) 2 and 3 might explain the restrictive EPO cell recruitment. This study shows that although HIF-2 can be clearly stabilized in deeper medullary fibroblasts by pharmacological PHD-inhibition, this is not sufficient to induce EPO in these cells. In contrast, genetic stabi

SUBMITTER: Firmke BKM 

PROVIDER: S-EPMC12487596 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature