Recalibrating differential gene expression by genetic dosage variance prioritizes functionally relevant genes.

ABSTRACT: Differential expression (DE) analysis is a widely used method for identifying genes that are functionally relevant for an observed phenotype or biological response. However, typical DE analysis includes selection of genes based on a threshold of fold change in expression under the implicit assumption that all genes are equally sensitive to dosage changes of their transcripts. This tends to favor highly variable genes over more constrained genes where even small changes in expression may be biologically relevant. To address this limitation, we have developed a method to recalibrate each gene's DE fold change based on genetic expression variance observed in the human population. The newly established metric ranks statistically differentially expressed genes, not by nominal change of expressi

SUBMITTER: Rentzsch P 

PROVIDER: S-EPMC12487816 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature