Human red blood cell ATP content and export under hypoxic and/or isocapnic storage conditions.

ABSTRACT:

Introduction

In some diseases driving or associated with anemia, red blood cell (RBC) transfusion conveys limited benefit, and only when the anemia is severe. The banking of RBCs alters key molecules and functions. Among these changes during blood banking, depletion of the allosteric effector 2,3-BPG (biphosphoglycerate) takes place in the first two to 3 weeks, while ATP depletion only becomes prominent in the fifth or sixth (i.e., final) weeks of storage. One approach to testing the significance of these changes is to test the effects in vitro and in vivo of stabilizing key molecules. We hypothesized that hypoxic RBC storage, which can stabilize RBC BPG and ATP generation, could in turn stabilize the ability of RBCs to export vasoactive ATP, an activity that modulate

SUBMITTER: Chen Y 

PROVIDER: S-EPMC12488716 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature