Cassini: streamlined and scalable method for in situ profiling of RNA and protein.

ABSTRACT: In the expanding field of spatial genomics, numerous methods have emerged to decode biomolecules in intact tissue sections. Advanced techniques based on combinatorial decoding can resolve thousands of features in a reasonable time but are often constrained by either the prohibitive costs associated with commercial platforms or the complexity of developing custom instruments. Alternatively, sequential detection methods, like single-molecule FISH, are easier to implement but offer limited multiplexing capability or signal amplification. Here, we introduce Cassini, an innovative approach for straightforward, cost-effective multiplexed measurements of mRNA and protein features simultaneously. Cassini leverages rolling circle amplification, known for its robust amplification and remarkable stab

SUBMITTER: Lapique N 

PROVIDER: S-EPMC12488881 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature