3D histology of human heart-forming organoids by X-ray phase-contrast tomography.

ABSTRACT: Three-dimensional (3D) imaging is crucial for elucidating the complex structure of organoid models which involve complex spatial cellular and tissue organization in 3D. While a variety of volume imaging methods, including novel light microscopy tools, are now well established to probe the cellular complexity of organoids in 3D, the gold standard for obtaining a precise morphological picture is histology, a traditionally 2D imaging technique that relies on slicing the specimen and therefore has severe limitations in scalability and volumetric imaging. X-ray phase-contrast tomography (XPCT) has emerged as an imaging modality capable of extending conventional histology into the third dimension. While it has been applied to various types of animal and human tissues, its applicability to organo

SUBMITTER: Komorowski K 

PROVIDER: S-EPMC12488953 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature