Super-Resolution Microscopy Reveals Alterations in Clathrin Structure in Human Cancer Tissue.

ABSTRACT: Super-resolution microscopy holds great promise for detailed structural analysis of proteins, yet its application in the investigations of protein structures in situ remains sparse. Clathrin-coated pit-mediated endocytosis (CME) plays a key role in human cancer. This study aimed to discover whether there are structural changes in clathrin pits in cancer. Immunofluorescence combined with super-resolution structured illumination microscopy (SR-SIM) on normal and cancerous prostate tissue was used to reveal novel details of clathrin structure and biology. Clathrin (heavy-chain) plaques and pits, expression of adaptor protein 2 (a clathrin adaptor protein), and epidermal growth factor receptor (a receptor target for CME) at nanometer scale in human tissue were observed in situ with immunofluor

SUBMITTER: Greig A 

PROVIDER: S-EPMC12489382 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature