Functionalization of lipid nanoemulsions with humanized antibodies using plug-and-play cholesterol anchor for targeting cancer cells.

ABSTRACT: Lipid nanoemulsions (NEs) are promising green nanocarriers for diagnostic and therapeutic applications, but their functionalization with biomolecules, such as antibodies, remains a challenge due to liquid nature of their core. Here, we developed an original plug-and-play strategy to graft an antibody (trastuzumab) at the surface of NEs, using components generally recognized as safe (GRAS). We synthesized a reactive 4-nitrophenyl carbonate of cholesterol (NPC-Chol) and a Biotin-PEG₃₀₀₀-Lysine linker, which can react within one-pot formulation to form an amphiphilic carbamate Biotin-PEG₃₀₀₀-Cholesterol. The cholesterol ensures anchorage of the linker, which effectively exposes biotin moiety at the surface of NEs for further antibody grafting using a biotin-neutravidin c

SUBMITTER: Boide-Trujillo VJ 

PROVIDER: S-EPMC12489389 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature