Click DNA ligation with deoxyribozyme.

ABSTRACT: Deoxyribozymes that ligate DNA will expand the reaction scope of DNA catalysis and are useful in the construction of DNA nanostructures. Herein, we report the first efforts to isolate a novel class of DNA ligase deoxyribozymes from a random sequence DNA pool by in vitro selection. The identified deoxyribozymes catalyze the intermolecular linear DNA-DNA ligation via the formation of unnatural triazole linkages between a 5' alkyne and a 3' azide. One remarkable click-ligating deoxyribozyme, named CLDz2, ligated DNA with an observed rate constant (kobs) up to 2.7 × 10-2 h-1 at 10 mM Mn2+ (pH 7.0, 30°C), with up to 40% yield in overnight incubations. CLDz2 is predicated to have a four-way, junction-like structure comprised of four short duplexes, three hairpin loops, and two main interhelical

SUBMITTER: Chang Y 

PROVIDER: S-EPMC12489470 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature