Detection of Bacterial Activity via Reduction of Prussian Blue Thin Films as Probed by Raman Spectroscopy.

ABSTRACT: Biomarker-free and in situ detection of microbes and microbial biofilms is an important challenge. Prussian Blue (PB) has emerged as a potential material for a variety of biological applications. It is safe, sustainable, and also versatile, being employed as nanocarriers for drug delivery, antidotes for Cs and Tl poisoning, in photothermal therapy, and for the detection of live-dead bacteria. In this work, the ability of electrodeposited Prussian Blue (PB) thin films to detect bacterial activity was investigated using Staphylococcus aureus biofilm growth on the PB surface. The conversion of PB to Prussian White (PW), the reduced form of PB, due to the metabolic activity of S. aureus, was tracked using Raman microspectroscopy, enabling both time- and spatially resolved effects

SUBMITTER: Baggio BF 

PROVIDER: S-EPMC12489613 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature