Integrating Single-Cell Transcriptomic Data of Metastatic Gastric Cancer to Construct a Tumor-Associated Neutrophil Signature and Clinical Validation in MSS/pMMR Patients.

ABSTRACT: Background: Single-cell sequencing technology has emerged as a powerful tool for investigating the tumor immune microenvironment, enabling a deeper understanding of the heterogeneity of immune cell populations within tumors. Tumor-associated neutrophils (TANs) have become a focal point in the study of the tumor immune microenvironment due to their involvement in tumorigenesis, progression, and drug resistance. Methods: We analyzed scRNA-seq data from metastatic gastric cancer (GC) and bulk RNA-seq data from multiple GC cohorts. Non-negative matrix factorization (NMF) was used to classify molecular subtypes, and a prognostic model was constructed using LASSO Cox regression. Key genes were validated by using qRT-PCR and immunohistochemistry. Molecular docking was utilized to sc

SUBMITTER: Yao L 

PROVIDER: S-EPMC12489636 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature