Biomethane Production from Stillage: The Role of Temperature, Initial Substrate Load, and Inoculum Variability.

ABSTRACT: Molasses stillage, with its high organic content and nutrient-rich composition, represents a promising feedstock for biogas production. This study systematically evaluated its biochemical methane potential (BMP) using two inocula (wastewater treatment plant vs agricultural biogas plant) across three temperatures (40, 50, 60 °C) and initial substrate load (ISL: 2, 5, 10 g·L_incolum ^-1). The wastewater inoculum achieved superior methane yields (0.262-0.477 N m³·kg_vs ^-1), peaking at 50 °C with a 22% increase over agricultural systems (0.192-0.378 N m³·kg_vs ^-1). 16S rRNA sequencing revealed the wastewater treatment plant inoculum's superior functional diversity, dominated by syntrophic Chloroflexota a

SUBMITTER: Vitez T 

PROVIDER: S-EPMC12489696 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature