Chemical Profiling of Wild and Commercial Tomato Leaves: Protocol Optimization, Insights into Differences in Chemical Profiles between Species and Developmental Stages.

ABSTRACT: The tomato is a globally important crop whose domestication has led to significant chemical changes that may reduce its natural defenses. This study compares the chemical profiles of wild (Solanum pimpinellifolium) and commercial (Solanum lycopersicum, cultivar BRS Iracema) tomato leaves across three developmental stages: seedling, vegetative, and flowering. We used optimized ultrasound-assisted extraction for nonvolatile compounds and headspace extraction for volatile compounds. We coupled these methods with UHPLC-q-TOF-MS/MS and GC-MS to investigate their chemical composition. Multivariate analysis revealed that domestication profoundly altered chemical profiles, particularly affecting glycosylated alkaloids, phenylpropanoids, and gibberellins. During the seedling stage,

SUBMITTER: Aguiar MCS 

PROVIDER: S-EPMC12489728 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature