Determination of Half-Maximal Inhibitory Concentration (IC₅₀) of Drugs Using Contrast Surface Plasmon Imaging on Gold-Coated Periodic Nanowires.

ABSTRACT: The half-maximal inhibitory concentration (IC₅₀) is a critical pharmacological parameter used to quantify a drug's potency in inhibiting biological functions. In this study, we present a novel strategy to evaluate the cytotoxicity of anticancer drugs on CL1-0 and A549 lung cancer cells, Huh-7 liver cancer cells, and MCF-7 breast cancer cells using contrast surface plasmon resonance (SPR) imaging with gold-coated periodic nanowire array sensors. The gold nanostructures, with a periodicity of 400 nm, produced a reflective SPR dip at 580 nm─positioned at the overlap between red and green channels of a color CCD sensor. The differential SPR response, captured through contrast imaging of red and green channels, reflected changes in cell adhesion. At predefined time points during cyto

SUBMITTER: Hou HS 

PROVIDER: S-EPMC12489899 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature