Identification of lipids and proteins associated with different heat treatments in sheep milk based on lipidomics and proteomics.

ABSTRACT: To fully investigate the effects of heat treatment on proteins and lipids in sheep milk, lipidomics and Astral-DIA proteomics combined with parallel reaction monitoring methods were used to analyze the changes of lipids and proteins after heat treatment at 65 °C for 30 min and 95 °C for 15 s. A total of 79 and 381 differential lipids and proteins were identified respectively. Lipidomics results show that Triacylglycerol (16:1_17:1_16:2) was unique in raw milk, whereas diacylglycerol (20:1_22:1) was unique in 95 °C group. Choline metabolism in cancer was the core metabolic pathway linked with heat treatment. Additionally, the collagen alpha-3(VI) chain protein (COL6A3), folate receptor-like domain-containing protein (FOLR1), and serpin domain-containing protein (JEQ12_008015) gradually disa

SUBMITTER: Zhu Z 

PROVIDER: S-EPMC12489914 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature