Single-cell transcriptomics of organoids reveals transcriptional control of germline stem cell fate by an E2F1-TFAP2C-SOX17 positive-feedback loop in Turner syndrome.

ABSTRACT:

Background

Turner syndrome is the most common sex chromosome abnormality in females and affects approximately 1-2 in 2500 girls and women. To date, no cure is available for the treatment of infertility in Turner syndrome patients with 45,XO.

Results

Here, we report an organoid approach for generating human germline stem cells and their somatic niche cells in the Turner syndrome. The niche provides an appropriate microenvironment for germline fate in the organoids. Single-cell transcriptomics reveals the dynamics of germline development from iPSCs to germline stem cells, together with their somatic niche. The germline stem cells follow a successive trajectory of four developmental states with featured molecular events, which determine the developmental fate of the germline st

SUBMITTER: Shang D 

PROVIDER: S-EPMC12490042 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature