Evaluating urine volume and host depletion methods to enable genome-resolved metagenomics of the urobiome.

ABSTRACT:

Background

The gut microbiome has emerged as a clear player in health and disease, in part by mediating host response to environment and lifestyle. The urobiome (microbiota of the urinary tract) likely functions similarly. However, efforts to characterize the urobiome and assess its functional potential have been limited due to technical challenges including low microbial biomass and high host cell shedding in urine. Here, to begin addressing these challenges, we evaluate urine sample volume (100 ml-5 mL) and host DNA depletion methods and their effects on urobiome profiles in healthy dogs, which are a robust large animal model for the human urobiome. We collected urine from seven dogs and fractionated samples into aliquots. One set of samples was spiked with host (canine) cells to

SUBMITTER: Lewis ZJ 

PROVIDER: S-EPMC12490163 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature