Dissolution Dynamic Nuclear Polarization of the ⁷⁷Se Nucleus.

ABSTRACT: ⁷⁷Se is a spin ½, low sensitivity nucleus with a natural abundance of 7.6%. Although ⁷⁷Se NMR is very useful in the characterization of selenium containing molecules including seleno-proteins, the detection of ⁷⁷Se is challenging in biological samples without enrichment. Therefore, the goal of this work was to establish whether the ⁷⁷Se signal could be enhanced in the liquid state by dissolution dynamic nuclear polarization (DNP) NMR without the need of enrichment. The dominant spin-lattice relaxation mechanism for ⁷⁷Se is via chemical shift anisotropy, which is highly dependent on the molecular symmetry. Here we tested three selenium compounds (sodium selenate, sodium selenite and selenocystine) with different molecular symmetries in

SUBMITTER: Suh EH 

PROVIDER: S-EPMC12490296 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature