Influence of lipid saturation on the structural properties of styrene maleic acid lipid nanoparticles (SMALPs).

ABSTRACT: Membrane bilayers are complex three-dimensional structures whose molecular events in the deep dimensions of membrane lipids are crucial for understanding membrane function. This study investigates the interaction of coexisting membrane domains in terms of hydrophobicity, alkyl chain order, and fluidity using Styrene Maleic Acid (SMA) copolymers as membrane mimics. We employed continuous wave electron paramagnetic resonance spectroscopy (CW-EPR) to characterize the structural dynamic properties of membrane domains without separation. Lipid-spin probe vesicles were prepared using phospholipids with varying degrees of saturation (DOPC, POPC, DMPC, and DSPC) and doxyl spin-labeled phospholipids at different depths (5, 12, and 16-doxyl PC) as membrane probes. These vesicles were titrated with t

SUBMITTER: Okorafor EA 

PROVIDER: S-EPMC12490428 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature