Identification of Novel Peptides Originated from Biodegradation of Microcystins Using UHPLC-HRMS/MS.

ABSTRACT: Biodegradation of toxic cyanobacterial cyclic peptides microcystins (MCs) by heterotrophic bacteria isolated from freshwater has gained global interest as an eco-friendly approach for water treatment. This study focused on elucidating the structures of MC-LR breakdown products generated by a group of five bacteria previously isolated from Lake Erie. Three isomers of a tetrapeptide NH₂-Adda-Glu-Mdha-Ala-OH (Adda-TetraP-1, 2, and 3) and a cyclic tripeptide NH₂-Adda-Glu-Mdha-OH (Adda-TriP) were identified during the MC-LR degradation process. Of these peptides, only linear Adda-TetraP-1 was described previously. UHPLC-high-resolution (HR)-MS and MS/MS data acquired for tetrapeptides and the tripeptide and their thiol derivatization showed that Adda-TetraP-2, Adda-TetraP-

SUBMITTER: Thenuwara SI 

PROVIDER: S-EPMC12490793 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature