Extracellular vesicles shed by macrophages co-cultured with <i>Lactiplantibacillus plantarum</i> No. 14 reduce adipocyte size in mice fed a high-fat diet.

ABSTRACT: Our previous study showed that the intragastric administration of Lactiplantibacillus plantarum No. 14 reduces adipocyte size in white adipose tissue of high-fat diet (HFD)-induced obese mice. We also suggested that the anti-inflammatory effect of L. plantarum No. 14 in white adipose tissues is mediated, at least in part, by circulating exosomes, a type of extracellular vesicle (EV). Therefore, the present study examined whether EVs mediate the adipocyte size-reducing effect of L. plantarum No. 14. Macrophages derived from mouse bone marrow were co-cultured with L. plantarum No. 14, and EVs were isolated from the culture supernatant by ultracentrifugation. C57BL/6J mice fed an HFD were intravenously administered the EVs 5 times a week for 7 weeks. We found that

SUBMITTER: Tsuruta T 

PROVIDER: S-EPMC12490871 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature