Genetic analysis of a β-1,3/1,4-glucan utilization gene cluster in <i>Segatella copri</i> JCM 13464^T.

ABSTRACT: Barley β-glucan (BGL), a dietary fiber composed of β1,3- and β1,4-linked glucose units, confers various health benefits, including anti-diabetic effects. A previous study reported that the anti-diabetic effect of BGL was associated with gut bacteria, particularly Segatella copri. Recently, a study using recombinant proteins revealed the biochemical characteristics of proteins encoded by the polysaccharide utilization locus 4 (PUL4), which is implicated in BGL assimilation. However, the precise physiological roles of PUL4 remain unclear. In this study, we used gene disruption in S. copri JCM 13464^T (=DSM 18205^T; also known as Prevotella copri CB7) to investigate the physiological functions of PUL4 under BGL-supplemented conditions. Deletion of p

SUBMITTER: Oyama M 

PROVIDER: S-EPMC12490936 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature