Comparative LC-MS/MS-based profiling of phytohormones: a unified analytical approach across diverse plant matrices.

ABSTRACT:

Introduction

Phytohormones are critical regulators of plant growth, development, and stress responses. Despite advancements in analytical techniques, profiling multiple phytohormones across various plant matrices using a standardized approach remains underexplored.

Methods

This study presents a unified LC-MS/MS analytical platform, employing consistent chromatographic and mass spectrometric conditions in combination with tailored matrix-specific extraction procedures, to profile and quantify key phytohormones. This includes abscisic acid (ABA), salicylic acid (SA), gibberellic acid (GA), and indole-3-acetic acid (IAA), across five plant matrices of significant agricultural and medicinal value. The method was validated for sensitivity, reproducibility, and matrix adaptability

SUBMITTER: Hakeem MK 

PROVIDER: S-EPMC12491226 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature