Stemness- and hypoxia-based prognostic stratification index reveals G6PD as a regulator of hypoxia-driven stemness in hepatocellular carcinoma.

ABSTRACT:

Background

The positive feedback loop between cancer stemness and the hypoxic microenvironment is a critical driver of hepatocellular carcinoma (HCC) progression. Analyzing their interaction in HCC is crucial to characterize immune microenvironment features, uncover molecular heterogeneity patterns, and develop targeted interventions.

Methods

The TCGA-LIHC cohort (n=340) were stratified through consensus clustering of stemness- and hypoxia-related genes (SHRGs) identified by one-class logistic regression and weighted gene co-expression network analyses. Subsequently, a stemness- and hypoxia-related prognostic index (SHRPI) was constructed using random forest, and Cox regression analyses, with its prognostic significance assessed in two other independent cohorts: our NC-LT co

SUBMITTER: Gao M 

PROVIDER: S-EPMC12491235 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature