Stool shed cell transcriptomics mirrors tumor biology and enables colorectal cancer diagnosis.

ABSTRACT: Screening and molecular characterization of human intestinal pathologies such as colorectal cancer (CRC) currently depends on colonoscopy, an invasive procedure associated with risks and poor adherence. A non-invasive method that captures host molecular changes could improve early detection and monitoring of intestinal diseases. Transcriptomic profiling of shed intestinal cells in stool has shown potential in neonates but is limited in adults by the dominance of bacterial RNA. To address this, we combined microbial ribosomal RNA (rRNA) depletion with unique molecular identifier (UMI)-based RNA sequencing to enrich and quantify human transcripts in stool. Applying this method to samples from 54 CRC patients and 24 healthy controls, we profiled thousands of human genes per sample. Stool-deri

SUBMITTER: Bahar Halpern K 

PROVIDER: S-EPMC12491572 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature