Direct shoot regeneration and gene expression profiling for optimized picroside-I biosynthesis in Picrorhiza Kurroa.

ABSTRACT: Picrorhiza kurroa, an endangered medicinal herb, is known for its bioactive iridoid glycosides, the Picrosides, which possess a range of pharmacological properties, including hepatoprotective, anti-inflammatory, and antioxidant activities. As demand for this valuable plant increases, in vitro tissue culture conservation efforts have become critical. We attempted in vitro tissue engineering-assisted direct shoot regeneration from leaf explants of P. kurroa using various growth hormone combinations, for enhanced metabolite production. MS media, admixed with 0.5 mg/L thidiazuron and 1.5 mg/L kinetin, bypassed the callogenesis phase while offering 83% shoot regeneration efficiency. High-performance liquid chromatography analyses with shoots elucidated a high picroside-I content of up to 9.7 µg

SUBMITTER: Shishodia SK 

PROVIDER: S-EPMC12491585 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature