Robustness of urinary extracellular vesicle-derived MiRNA profiles over multiple days and the impact of urine concentration.

ABSTRACT: Urinary extracellular vesicle (uEV)-derived microRNAs (miRNAs) have emerged as promising noninvasive biomarkers for disease prediction. However, fluctuations in urine concentration throughout the day may influence miRNA abundance and profiles, potentially limiting their clinical utility. To ensure the broader applicability of uEV-derived miRNAs in disease prediction, it is essential that their profiles remain stable regardless of collection time. In this study, we systematically examined factors influencing miRNA abundance in uEVs and assessed the stability of miRNA profiles. We collected serial urine samples from healthy individuals over three days and performed small RNA sequencing to analyze uEV-derived miRNA profiles in relation to various urine concentration parameters. Total miRNA co

SUBMITTER: Satomura A 

PROVIDER: S-EPMC12491611 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature