External-Force-Offset Effects of ECM Coating Layers on hMSCs Subjected to External Physical Force.

ABSTRACT: Mesenchymal stem cells (MSCs) used for cell-delivery-based therapy also undergo considerable external stresses upon entering the recipient site in the body. Here, we sought to develop a cell-protective barrier on the MSC surface that protects against stress-induced damage from physical external stresses. The barrier was fabricated from gelatin and hyaluronic acid (HyA) using a layer-by-layer (LbL) technique. In addition to assessing the stability and biological properties of extracellular matrix (ECM)-coated human bone marrow-derived MSCs (hMSCs) produced using the LbL, we also evaluated the cell-protective effects of this coating against 2 external stresses: low-attachment conditions and mechanical force induced by injection. Cell biological and morphological surface changes accompanying

SUBMITTER: Park CY 

PROVIDER: S-EPMC12491781 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature