Proteome-Wide 4-Hydroxy-2-Nonenal Signature of Oxidative Stress in the Marine Invasive Tunicate Botryllus schlosseri.

ABSTRACT: The ascidian Boytryllus schlosseri is a marine chordate that thrives under conditions of anthropogenic climate change. The B. schlosseri expressed proteome contains unusually high levels of proteins adducted with 4-hydroxy-2-nonenal (HNE). HNE represents a prominent posttranslational modification resulting from oxidative stress. Prior to this study, which identified 1052 HNE adducted proteins in B. schlosseri by LCMS, HNE protein modification has not been determined in any marine species. Adducted residues were ascertained for 1849 HNE modifications, 1195 of which had a maximum amino acid localization score. Most HNE modifications were at less reactive lysines (rather than more reactive cysteines). HNE prevalence on most sites was high, suggesting that B. schlosseri experiences and tolerat

SUBMITTER: Kultz D 

PROVIDER: S-EPMC12491926 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature